Generator

Part:BBa_K235014

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)


[K145303][K235001] (ribokey-mediated GFP generator)

This GFP generator has an intermediate step using a ribokey and ribolocked RBS. It could be used as a control to compare against K235013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 964
    Illegal NheI site found at 987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 998


[edit]
Categories
Parameters
None